- Applied Biosystems StepOnePlus Real-Time PCR system
- Power cable
- 120-day warranty
After its initial disinfection and inspection, our technicians tested the Applied Biosystems StepOnePlus real-time PCR system with the following results:
- Ran cycle performance test: PASSED
- Ran optics verification test: PASSED
- Installed shipping block and performed shutdown: PASSED
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Manufacturer
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Applied Biosystems
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Product line
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StepOnePlus
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Ref. No.
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4376592
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Serial No.
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2720012067
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Mfg. date
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2018-08-28
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Format
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96-well plate, 8-tube strips, 0.1ml tubes
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Capacity
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96 x 0.1 ml tubes, 12 x 8-tube strips, 1 x 96-well plate
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Sample ramp rate
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Fast mode: ±2.2°C/s, Standard mode: ±1.6°C/s
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Temperature range
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4 to 100°C
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Thermal accuracy
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0.25°C (35°C to 95°C) of display temperature
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Sensitivity
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1 copy
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Reaction volume range
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10-30µl (Standard curve experiments: 40µl in Standard mode is validated
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Dynamic range
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Linear dynamic range greater than 9 log units (detection)
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High-throughput compatibility
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Multiplexing
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Passive reference dye
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ROX (separate tube), No ROX, ROX (pre-mixed)
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Calibrated dye
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VIC, SYBR Green I, ROX, JOE, NED, FAM, TAMRA
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Detection method
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SYBR, Primer-Probe Detection
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Optics
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4 emission filters, Blue LED excitation source, photodiode
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Reaction speed
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Fast, Standard
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Run time
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<2 hrs/run (Standard mode), <40 min/run (Fast mode)
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Thermal cycling system
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Peltier-based system
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Display type
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LCD touchscreen
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Electrical requirements
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120V, 50/60Hz, 900W
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Dimensions (W x D x H)
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9.7 x 19 x 20.2 in
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Weight
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52 lbs (24 kg)
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