- Molecular Devices SpectraMax iD3 Multi-Mode Microplate Reader
- Computer with SoftMax Pro 7 Software Installed
- Monitor, Keyboard, and Mouse
- Battery Backup
- Tools and Transport Locks
- Communication Cables
- Power Cables
- 90-Day Warranty
After its initial disinfection and inspection, our technicians tested the Molecular Devices SpectraMax iD3 multi-mode microplate reader with the following results:
- Reader power and initialization: PASSED
- Reader and computer connection: PASSED
- Ran SpectraMax standard plate; results within specification: PASSED
- Plate loading and unloading: PASSED
- Shaking: PASSED
- Temperature control: PASSED
Notice: Transport locks will be installed when this instrument arrives at your location.
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Manufacturer
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Molecular Devices
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Model
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SpectraMax iD3
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Serial No.
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37 370-3636
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Mfg. date
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2018-12-20
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Plate formats
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6, 12, 24, 48, 96, 384-well plates ANSI/SLAS conformant
Maximum height: 22 mm
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Reading capability
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Plates and cuvettes (with adapter) SpectraCuvette Adapters without a sticker have a plate height of 24 mm and cannot be used in the SpectraMax iD3 or SpectraMax iD5.
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Robotic compatible
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Yes
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Shake
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Orbital, double orbital, and linear
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Temperature control
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5°C (7.2°F) above ambient up to 66°C (150.8°F)
At temperatures ranging from 55°C (131°F) up to 66°C (150.8°F) ambient temperature of 25°C (77°F) is required.
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Chamber temperature
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<5°C ambient 25°C, under DutyCycle of: FL Top, 400ms/well, complete 384-wells, pause to achieve 10 min cycle time, eject, wait 30 sec, load.
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Temperature uniformity
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±0.75°C (1.35°F)
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Temperature accuracy
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±1°C (1.8°F) at 37°C (98.6°F) Set Point
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Wavelength selection
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1.0 nm increments
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Ambient operating temperature
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15°C to 40°C (59°F to 104°F)
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Ambient storage temperature
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-5°C to 40°C (23°F to 104°F) continuous;
-20°C to 50°C (-4°F to 122°F) transient (up to 10 hours)
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Humidity restrictions
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15% to 75% (non-condensing) at 30°C (86°F)
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Altitude restrictions
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Up to 2000 m (6562 ft)
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Air pressure restrictions
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54 kPa to 106 kPa (7.8 PSI to 15.4 PSI)
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Sound pressure level
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Maximum sound pressure: 73 dBA
Maximum sound pressure at one meter: 68 dBA
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Installation category
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II
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Pollution degree
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2
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Data connection
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One ethernet port
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NFC antenna reader/writer
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SANGOMA-MSMA 2V5 13.56 Mhz Multi Standard - Multi Antenna Reader/Writer
Contains FCC ID: 2AKHW-SANGMSMA4 Contains IC: 22202-SANGMSMA4
Changes or modifications made to this equipment not expressly approved by the party responsible for compliance may void the FCC authorization to operate this equipment.
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Front clearance
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4.33 in (11 cm) for plate drawer
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Rear clearance
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7.9 to 11.8 in (20 to 30 cm) between the rear of the instrument and the wall for ventilation and cable disconnects
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Power rating
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100-240V~, 50-60Hz, 2A
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Dimensions (W x D x H)
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20.94 x 23.54 x 15.79 in (53.2 x 59.8 x 40.1 cm)
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Weight
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88.1 lb (40 kg)
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Read Mode
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96-Wells
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384-Well
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Absorbance
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0:25
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1:25
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Fluorescence Intensity* (10 msec integration time)
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0:17
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0:53
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Luminescence (100 msec integration time 96-well, 40 msec integration time 384-well plate)
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0:26
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1:01
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*For >65°C, minimum 25°C ambient temperature is required
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Item
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Description
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Wavelength range
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230 - 1000 nm
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Wavelength selection
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Monochromator tunable in 1 nm increments
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Wavelength bandwidth
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4.0 nm full width half maximum
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Wavelength accuracy
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±2.0 nm across wavelength range
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Wavelength repeatability
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±1.0 nm
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Photometric range
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0 - 4.0 OD
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Photometric resolution
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0.001 OD
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Photometric accuracy
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< ±0.010 OD ±1.0%, 0 - 3 OD VIS 0 - 3 OD UV
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Photometric precision (repeatability)
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< ±0.003 OD ±1.0%, 0 - 3 OD VIS 0 - 3 OD UV
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Stray light
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≤ 0.05% at 230 nm, 280 nm
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Photometric stabilization
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Instantaneous
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Photometric drift
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None (continuous referencing of monochromatic input)
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Calibration
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Automatic before every endpoint read and before the first kinetic read
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Optical alignment
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None required
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Photodetectors
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Silicon Photodiode
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Item
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Description
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Wavelength range
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EX 250 - 830 nm
EM 270 - 850 nm
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Wavelength selection
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Monochromators tunable in 1.0 nm increments
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Wavelength accuracy
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±2.0 nm
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Wavelength repeatability
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±1.0 nm
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Bandwidth (EX/EM)
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EX: 15 nm
EM: 25 nm
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Number of excitation/emission pairs per plate
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4
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Dynamic range
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>6 logs
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Sensitivity top read mono*
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Fluorescein
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96-wells
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4 pM - Guaranteed
1 pM - Optimized
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384-wells
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6 pM - Guaranteed
1 pM - Optimized
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Sensitivity bottom read mono*
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Fluorescein using glass bottom Greiner Sensoplate glass bottom multiwell plates
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96-wells
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10 pM - Guaranteed
2 pM - Optimized
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384-wells
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10 pM - Guaranteed
2.5 pM - Optimized
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System validation
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Self-calibrating with built-in fluorescence calibrators
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Light source
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High power xenon flash lamp
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Average lamp lifetime
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1 billion flashes or 2 years normal operation
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Item
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Description
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Wavelength selection
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Choice of simultaneous detection of All Wavelengths or selection in 1.0 nm increments
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Wavelength range
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300 - 850 nm
300 - 650 nm for “All Wavelengths” setting
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Wavelength accuracy
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±2.0 nm
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Wavelength repeatability
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±1.0 nm
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Dynamic range
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>7 decades
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Sensitivity top read*
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Perkin Elmer ATPlite 1step Luminescence Assay System
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96-well
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10 pM - Guaranteed
2 pM - Optimized
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384-well
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20 pM - Guaranteed
4 pM - Optimized
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Crosstalk
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<0.1% in white 96-well half area plate
<0.2% in white 384-well Costar small volume
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*For properly functioning, operating, and maintained equipment.
