Used Eppendorf Microcentrifuges

The Eppendorf 5417C reaches 14,000 rpm, which suits applications that require elevated centrifugal force, such as pelleting dense cellular fractions, nucleic acid-protein complex precipitation and protocols that specify g-force requirements above the range of a standard unit.

Eppendorf designed the 5417C platform for high-speed work with 2.0 mL tubes. Verify the rotor type and g-force rating in the individual product listing before purchase.

Yes, Eppendorf 5417C centrifuges handle high-speed microcentrifugation well. The 5417C platform supports speeds up to 14,000 rpm across most listed configurations. These speeds suit a broad range of common high-speed protocols, such as spin-column nucleic acid purification, protein precipitation and cell debris removal.

The F45-30-11 fixed-angle rotor, included with some listed units, holds tubes at 45 degrees, which supports efficient pellet formation at these speeds. Confirm that the specific unit's rotor and tube capacity align with your protocol before purchase.

The Eppendorf model with the highest listed centrifugal force in the current inventory is the 5417C at 25,000 x g. This centrifugal force level covers applications that demand very high pelleting efficiency, such as pelleting dense cellular fractions, bacterial cell harvest from small volumes and precipitation of large protein complexes that require higher forces. 

Yes, fixed-angle rotor configurations appear across several Eppendorf microcentrifuge units in the current inventory. Examples from the current listings include F45-30-11 rotors. A fixed-angle rotor holds tubes at a set angle, directing centrifugal force toward the tube wall and base to produce compact, well-defined pellets.

This design suits high-speed protocols like spin-column nucleic acid purification, protein pellet collection and cell debris separation, where tightly formed pellets and run-to-run consistency matter most. Rotor models and included accessories vary by unit, so review each product listing to confirm which rotor comes with the unit and whether it aligns with your protocol's tube size and speed requirements.

The tube type and rotor capacity details to confirm before you choose an Eppendorf microcentrifuge include tube volume compatibility, the number of rotor positions and whether the rotor requires adapters for your specific tube format.

Tube volume compatibility matters because not every rotor accepts every tube size. Some microcentrifuge rotors support tubes that require an adapter, and not all rotor configurations include one.

The number of rotor positions determines how many samples you can process per run, so confirm the position count fits your typical batch size. You should also check that the rotor's maximum rated speed matches your protocol's requirements, as speed limits can vary across rotor models even within the same centrifuge platform.