- BioTek Synergy H1 Hybrid Multimode Microplate Reader (H1MFG)
- PC with Gen5 Software Installed
- Monitor, Keyboard, and Mouse
- USB Data Cable
- Power Adapter
- Power Cables
- 90-Day Warranty
Notice: This reader does not have filters so Luminescence is not possible until filters are acquired. Filters can be obtained from BioTek. This reader has monochromator based absorbance.
After its initial disinfection and inspection, our technicians tested the BioTek Synergy H1 hybrid reader with the following results:
- Microplate reader initialization: PASSED
- Microplate reader and PC connection: PASSED
- Ran our ABS Standard plate with consistent results: PASSED
- Spectral and Absorbance scans: PASSED
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Manufacturer
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BioTek Instruments
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Model
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Synergy H1
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Model No.
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H1MFG
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Serial No.
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21062210
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Detection modes
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UV-Vis absorbance
Fluorescence intensity (top and bottom)
Luminescence
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Microplate types and lab glassware
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6- to 384-well plates, Petri and cell culture dishes
Take 3 micro-volume plates
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Absorbance
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Light source
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Xenon flash
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Detector
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Photodiode
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Wavelength selection
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Monochromator
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Wavelength range
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230-999 nm, 1 nm increment
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Bandwidth
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<4 nm (230-285 nm), <8 nm (>285 nm)
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Dynamic range
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0 - 4.0 OD
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Resolution
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0.0001 OD
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Pathlength correction
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Yes
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Monochromator wavelength accuracy
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±2 nm
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Wavelength repeatability
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±0.2 nm
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Reading speed (kinetic)
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96 wells: 11 seconds
384 wells: 22 seconds
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Fluorescence intensity
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Lamp
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Xenon Flash
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Wavelength range
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250-700 nm ex
250-700 nm em
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Bandpass
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≤18 nm (excitation and emission)
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Sensitivity
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Sodium Fluorescein in phosphate-buffered saline (PBS)
DL ≤20 pM, top or bottom read
Excitation 485nm, Emission 528nm
Methylumbelliferone (MUB) in carbonate-bicarbonate buffer (CBB)
DL ≤0.16 ng/mL, top read
Excitation 360 nm, Emission 460 nm
Propidium Iodide (PI) in PBS
DL ≤62.5 ng/mL, bottom read
Excitation 485 nm, Emission 645 nm
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Reading speed
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96 wells: 11 seconds
384 wells: 22 seconds
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Luminescence
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Sensitivity
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≤ 75 amol/well flash ATP in a 96-well plate
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General
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Temperature control
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Four zone incubation to 45°C; variation +0.2°C at 37°C with condensation control
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Shaking
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Linear, orbital, double orbital
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Control
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Gen5 software
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Power rating
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100-240VAC, 50/60Hz
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Dimensions (W x D x H)
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14.75 x 18.25 x 13 in
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Weight
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<55 lb
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