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Manufacturer
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Agilent Technologies, Inc
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Model
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Synergy Neo2
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Product No.
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N2SMA-SN
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Serial No.
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22042012
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Mfg. date
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2022-04-20
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Detection modes
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UV-Vis absorbance
Fluorescence intensity
Luminescence
Fluorescence polarization
Time-resolved fluorescence
Alpha
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Read methods
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End point, kinetic, spectral scanning, well-area scanning
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Microplate types
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6- to 1536-well plates
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Other labware supported
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Petri and cell culture dishes
Agilent BioTek Take3 microvolume plates
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Temperature control
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4-Zone Incubation to 70°C with Condensation Control
±0.2°C at 37°C
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Shaking
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Linear, orbital, double orbital
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Software
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Agilent BioTek Gen6 data analysis software
Agilent BioTek Gen5 Secure software enables 21 CFR Part 11 compliance (option)
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Automation
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Agilent BioTek BioStack microplate stacker and third-party automation compatible
Agilent BioTek BioSpa 8 automated incubator compatible
Agilent BenchCel microplate handler compatible
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CO2 and O2 control (Option)
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Range: 0-20% (CO2); 1-19% (O2)
Control resolution: ±0.1% (CO2 and O2)
Stability: ±0.2% at 5% CO2; ±0.2% at 1% O2
Models for CO2/O2, or CO2 only, are available
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Barcode reader
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1D and 2D camera-based scanner
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Absorbance
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Light source
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Xenon flash
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Detector
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Photodiode
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Wavelength selection
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Monochromator
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Wavelength range
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230-999 nm, 1nm increments
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Monochromator bandwidth
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<3 nm (230-285 nm), <6 nm (>285 nm)
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Dynamic range
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0-0.4 OD
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Resolution
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0.0001 OD
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Path length correction
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Yes
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Monochromator wavelength accuracy
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±2 nm
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Monochromator wavelength repeatability
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±0.2 nm
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OD accuracy
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<1% at 2.0 OD
<3% at 3.0 OD
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OD linearity
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<1% from 0-3.0 OD
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OD repeatability
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<0.5% at 2.0 OD
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Stray light
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0.03% at 230 nm
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Reading speed (kinetic)
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96 wells: 6 s
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Fluorescence Intensity
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Light source
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Xenon flash
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Detector
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PMT
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Wavelength selection
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Quad monochromators (top/bottom)
Filters (top/bottom)
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Wavelength range
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Monochromators: 250-700 nm (850 nm option)
Filters: 200-700 nm (850 nm option)
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Monochromator bandwidth
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Variable, from 3 to 50 nm in 1 nm increments
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Dynamic range
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7 decades
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Sensitivity
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Filters:
Fluorescein 0.2 pM (4 amol/well, 20 μL, 384-well low-volume plate)—top
Fluorescein 1 pM (10 amol/well, 10 μL, 1536-well plate)—top
Fluorescein 1 pM (0.1 fmol/well, 100 μL, 384-well plate)—bottom
Quad monochromator:
Fluorescein 2 pM (40 amol/well, 20 μL, 384-well low-volume plate)—top
Fluorescein 2.5 pM (0.25 fmol/well, 100 μL, 384-well plate)—bottom
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Reading speed (kinetic)
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96 wells: 6 s
384 wells: 11 s
1536 wells: 25 s
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Luminescence
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Wavelength range
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300-700 nm
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Dynamic range
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>6 decades
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Sensitivity
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5 amol ATP typical (384-well low-volume plate)
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Fluorescence Polarization
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Light source
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Xenon flash
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Detector
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PMT
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Wavelength selection
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Filters
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Wavelength range
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280-700 nm (850 nm option)
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Sensitivity
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1 mP standard deviation at 1 nM fluorescein
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Time-Resolve Fluorescence
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Light source
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Xenon flash or TRF laser (option)
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Detector
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Dual PMT or single PMT (option)
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Wavelength selection
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Filters (top/bottom)
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Sensitivity
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With TRF laser: 5 fM (384-well low-volume plate)
With Xenon flash lamp: 40 fM (384-well low-volume plate)
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Alpha Detection
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Light source
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100 mW 680 nm laser
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Detector
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PMT
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Wavelength selection
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Filters (top)
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Sensitivity
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100 amol LCK peptide (384-well low-volume plate)
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Read speed
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96 wells: 30 s
384 wells: 1 min 50 s
1536 wells: 7 min 20 s
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Reagent Dispensers
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Supported detection modes
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All modes
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Number
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2 syringe pumps
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Supported labware
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6- to 384-well plates, Petri dishes
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Dead volume
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1.1 mL, with backflush
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Dispense volume
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5-1,000 µL in 1 µL increments
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Dispense accuracy
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±1 µL or 2%
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Dispense precision
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≤2% at 50-200 µL
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Physical characteristics
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Power rating
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24VDC
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Power consumption
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250W max.
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Dimensions (W x D x H)
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Without TRF laser:
15.4 x 20.7 x 16.1 in (39.2 x 52.5 x 40.8 cm)
With TRF laser:
15.4 x 24.2 x 16.1 in (39.2 x 61.4 x 40.8 cm)
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Weight
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Without TRF laser: 78 lb (35.4 kg)
With TRF laser: 100 lb (45.3 kg)
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