- Thermo Scientific TSQ Endura Triple-Stage Quadrupole Mass Spectrometer 230V
- Thermo Scientific Vanquish Column Compartment/Oven H (VH-C10-A)
- Thermo Scientific Vanquish Split Sampler HT (VH-A10A) (6850.1915)
- Thermo Scientific Vanquish Binary Pump H Quaternary (VH-P10-A)
- Windows Workstation with Monitor, Keyboard, and Mouse
- Divert/Inject Valve Module with Cables
- Chemyx Fusion 101 Syringe Pump
- Peak Scientific NM32LA Nitrogen Generator 230V
- Sogevac SV65 BIFC Pump
- Ethernet Cables
- Power Cables
- 90-Day Warranty
Notice: The above list is an overview and does not include everything that comes with this system. Continue reading to see a complete breakdown of the included items and a section on testing performed to verify functionality.
- Thermo Scientific Xcalibur
- Thermo Scientific TraceFinder
- Thermo Scientific Tune/Instrument Control
- Chromeleon (installed but license is required for operation)
- TSQ Endura mainframe (serial: TQH E1 0464) — triple-quadrupole MS
- API source options: H-ESI / HESI-II, and APCI (includes ion transfer tube + ion sweep cone)
- Divert/Inject valve module with cables
- Syringe pump (for infusion/tuning) with power supply (Chemyx fusion 101)
- MS power cord(s) (230 Vac), region-specific
- Ethernet cable(s), Cat5e shielded, ≤3 m (for PC/control)
- Collision gas line from MS to argon regulator + fitting
- Nitrogen gas line from source to source interface + fitting
- Vanquish Split Sampler module (cat. no. 6850.1915)
- Pump module: Quaternary
- Column compartment/oven
- System interconnect cables (CAN/ethernet/control for Vanquish stack).
- LC power cords
- Bottle tray with caps & frits
- Autosampler racks/trays: 54 x 12 mm OD vials ( 22641.5 mL), 96 x 1.6, 7 and 8 mm OD vials ( 22641.2 mL), 24 x 15 mm OD vials ( 22644 mL), Deep well plates (96 and 384)
- Start/ready contact-closure cable (LC↔MS), if not using software handshake
- UHP & tubing to MS.
- Nitrogen (source/aux gas), HP, with PFA line to MS
- Lab exhaust for the forepump outlet and solvent waste trap vents
- Electrical outlets (typical):
- TSQ Endura: 230 Vac, ~5 A (1 outlet)
- Forepump: 230 Vac, ~8 A (1–2 outlets, 1 pump for Endura)
- PC/monitor/switch: 100–240 Vac (separate outlets)
- LC stack: per module (120/230 Vac). Total outlets commonly ≥10 for a single-forepump build
- Peek or stainless steel capillaries for LC→MS transfer
- Syringes (Hamilton) for direct infusion
- Waste containers/tubing with compatible caps
- Spare ion transfer tube & sweep cone (Endura)
- Spare forepump oil + exhaust mist filter
- Replacement autosampler needles/seals and sample loops (Vanquish Split Sampler)
- Pump seals/check valves (LC)
- Fuses (MS/LC)
- Autosampler confirmed functional through manual injection: PASSED
- LC system confirmed functional; flow path and divert valve operating correctly: PASSED
- Mass Spectrometer confirmed functional; capable of detecting analytes: PASSED
- Method Development substantially completed; ready for use in automated runs: PASSED
Notice: Continue reading for more details of our testing performed to verify functionality.
- A manual injection was performed to test the autosampler’s functionality.
- The needle was observed dipping correctly into the sample solution.
- Injection confirmed that the autosampler syringe system was operational and capable of aspirating/dispensing liquid.
- The LC pump and flow system were engaged and confirmed to be functional.
- Flow through the column and divert valve was verified, showing that the sample path to the mass spectrometer was intact.
- No leaks or pressure irregularities were observed, confirming the LC system’s stability.
- The TSQ Endura mass spectrometer was engaged and tested in operating mode.
- Signal response confirmed that the MS was detecting and recording ion events appropriately.
- This validated that the instrument was capable of receiving and processing analyte input from the LC system.
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Manufacturer
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Thermo Fisher Scientific
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Model
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TSQ Endura
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Serial No.
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TQH-E1-0464
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Mass range
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m/z 10-3400
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Resolution
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Q1 and Q3 adjustable to 0.4 Da peak width (FWHM) across the entire mass range
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Scan rate
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15,000 amu/second at a resolution of 2 FWHM
500 SRM/second (for any resolution from 0.4 through 2.0 FWHM)
25msec polarity switching
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Mass stability
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Mass assignment will be within ±0.05 Da over a 24 hour period. The laboratory room temperature must be maintained between 18–27 °C (65–81 °F). The room temperature may not change by more than 5 °C (9 °F) during this period.
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Positive Electrospray (HESI)
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A 2 µL injection of a 500 fg/µL reserpine solution will produce a minimum signal-to-noise ratio of 80,000:1 for the transition of the protonated molecule at m/z 609.3 to the fragment ion at m/z 195.1 when operated in selected-reaction monitoring (SRM) mode with Q1 and Q3 resolution set to 0.4 and 0.7 Da FWHM respectively.
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Atmospheric Pressure Chemical Ionization (APCI)
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A 2 μL loop injection of a 500 fg/μL reserpine solution will produce a minimum signal-to-noise ratio of 25,000:1 for the transition of the protonated molecular ion at m/z 609.3 to the fragment ion at m/z 195.1 when operated in selected reaction monitoring (SRM) mode with Q1 and Q3 resolution both set to 0.7 Da FWHM.
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Negative Electrospray (nESI)
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A 2 μL loop injection of a 500 fg/uL chloramphenicol solution will produce a minimum signal-to-noise ratio of 80,000:1 for the transition of the deprotonated molecular ion at m/z 321.0 to the fragment ion at m/z 152.0 when operated in selected reaction monitoring mode (SRM) with Q1 and Q3 resolution set to 0.4 and 0.7 Da FWHM, respectively.
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Gas requirements
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Collision gas: 99.995% pure argon
Collision gas supply pressure: 135 ± 70 kPa (20 ± 10 psig)
Sheath/aux/sweep gas: 99% pure nitrogen
Sheath/aux/sweep gas supply pressure: 690 ± 140 kPa (100 ± 20 psig)
Maximum sheath gas consumption: ≈20 L/min
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Environment requirements
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Functional temperature range: 15–27 °C (59–81 °F)
Optimal temperature range: 18–21 °C (65–70 °F)
Heat output: 1550 W (5400 Btu/h)
Total system heat output: 4420 W (15,380 Btu/h)
Particulate matter: <3,500,000 particles per cubic meter of air (<100,000 particles of >5 µm diameter per cubic foot of air)
Relative humidity: 20% to 80%, without condensation
Floors must be free of vibration
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Power consumption
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2300VA
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Power rating
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230V~, 50/60Hz, 10.0A max
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Dimensions (W x D x H)
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30 x 33 x 27 in (76 x 84 x 68 cm)
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Weight
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275 lb (125 kg)
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Manufacturer
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Thermo Fisher Scientific
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Device
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Column Compartment H
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Part No.
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VH-C10-A
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Serial No.
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6301071
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Mfg. date
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2016-11-11
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Power consumption
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326VA
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Power rating
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100-240V~, 50/60Hz, 4AT
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Dimensions (W x D x H)
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4.4 x 16.5 x 27.6 in (11.1 x 42 x 70 cm)
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Weight
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28.7 lb (13 kg)
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Manufacturer
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Thermo Fisher Scientific
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Device
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Split Sampler HT
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Part No.
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VH-A10-A
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Serial No.
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8302664
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Mfg. date
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2016-04-21
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Power consumption
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550VA
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Power rating
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100-240V~, 50/60Hz, 5AT
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Dimensions (W x D x H)
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16.5 x 11.4 x 24.4 in (42 x 29 x 62 cm)
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Manufacturer
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Thermo Fisher Scientific
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Device
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Binary Pump H
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Part No.
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VH-P10-A
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Serial No.
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8302653
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Mfg. date
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2016-04-21
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Power consumption
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550VA
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Power rating
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100-240V~, 50/60Hz, 4AT
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Manufacturer
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Peak Scientific
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Model
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NM32LA
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Serial No.
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A16-02-112
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Gas type
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Nitrogen
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Integrated compressor
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Yes
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Application types
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LC-MS or LC-MS/MS
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Max gas flow
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32L/min
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Max output pressure
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100psi/6.9bar
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Pressure gauges
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1
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Gas outlets fitting
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¼" BSP
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Start up time
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30 mins
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Heat output
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5729 BTU
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Max operating temperature
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30°C (86°F)
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Particles
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<0.01µm
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Noise level
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54 dBA @ 1m
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Accreditations
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CSA, FCC, CE
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Power rating
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~230V, 50/60Hz, 7A
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Power consumption
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1680 VA
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Dimensions (W x D x H)
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23.6 x 29.5 x 28.1 in (60 x 75 x 71.3 cm)
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Weight
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226.6 lb (103 kg)
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