Agilent BioTek Synergy Neo2 Multimode Microplate Reader

Manufacturer

Agilent Technologies, Inc

Model

Synergy Neo2

Product No.

N2SMA-SN

Serial No.

22042012

Mfg. date

2022-04-20

Detection modes

UV-Vis absorbance
Fluorescence intensity
Luminescence
Fluorescence polarization
Time-resolved fluorescence
Alpha

Read methods

End point, kinetic, spectral scanning, well-area scanning

Microplate types

6- to 1536-well plates

Other labware supported

Petri and cell culture dishes
Agilent BioTek Take3 microvolume plates

Temperature control

4-Zone Incubation to 70°C with Condensation Control
±0.2°C at 37°C

Shaking

Linear, orbital, double orbital

Software

Agilent BioTek Gen6 data analysis software
Agilent BioTek Gen5 Secure software enables 21 CFR Part 11 compliance (option)

Automation

Agilent BioTek BioStack microplate stacker and third-party automation compatible
Agilent BioTek BioSpa 8 automated incubator compatible
Agilent BenchCel microplate handler compatible

CO2 and O2 control (Option)

Range: 0-20% (CO2); 1-19% (O2)
Control resolution: ±0.1% (CO2 and O2)
Stability: ±0.2% at 5% CO2; ±0.2% at 1% O2
Models for CO2/O2, or CO2 only, are available

Barcode reader

1D and 2D camera-based scanner

Absorbance

Light source

Xenon flash

Detector

Photodiode

Wavelength selection

Monochromator

Wavelength range

230-999 nm, 1nm increments

Monochromator bandwidth

<3 nm (230-285 nm), <6 nm (>285 nm)

Dynamic range

0-0.4 OD

Resolution

0.0001 OD

Path length correction

Yes

Monochromator wavelength accuracy

±2 nm

Monochromator wavelength repeatability

±0.2 nm

OD accuracy

<1% at 2.0 OD
<3% at 3.0 OD

OD linearity

<1% from 0-3.0 OD

OD repeatability

<0.5% at 2.0 OD

Stray light

0.03% at 230 nm

Reading speed (kinetic)

96 wells: 6 s

Fluorescence Intensity

Light source

Xenon flash

Detector

PMT

Wavelength selection

Quad monochromators (top/bottom)
Filters (top/bottom)

Wavelength range

Monochromators: 250-700 nm (850 nm option)
Filters: 200-700 nm (850 nm option)

Monochromator bandwidth

Variable, from 3 to 50 nm in 1 nm increments

Dynamic range

7 decades

Sensitivity

Filters:
Fluorescein 0.2 pM (4 amol/well, 20 μL, 384-well low-volume plate)—top
Fluorescein 1 pM (10 amol/well, 10 μL, 1536-well plate)—top
Fluorescein 1 pM (0.1 fmol/well, 100 μL, 384-well plate)—bottom

Quad monochromator:
Fluorescein 2 pM (40 amol/well, 20 μL, 384-well low-volume plate)—top
Fluorescein 2.5 pM (0.25 fmol/well, 100 μL, 384-well plate)—bottom

Reading speed (kinetic)

96 wells: 6 s
384 wells: 11 s
1536 wells: 25 s

Luminescence

Wavelength range

300-700 nm

Dynamic range

>6 decades

Sensitivity

5 amol ATP typical (384-well low-volume plate)

Fluorescence Polarization

Light source

Xenon flash

Detector

PMT

Wavelength selection

Filters

Wavelength range

280-700 nm (850 nm option)

Sensitivity

1 mP standard deviation at 1 nM fluorescein

Time-Resolve Fluorescence

Light source

Xenon flash or TRF laser (option)

Detector

Dual PMT or single PMT (option)

Wavelength selection

Filters (top/bottom)

Sensitivity

With TRF laser: 5 fM (384-well low-volume plate)
With Xenon flash lamp: 40 fM (384-well low-volume plate)

Alpha Detection

Light source

100 mW 680 nm laser

Detector

PMT

Wavelength selection

Filters (top)

Sensitivity

100 amol LCK peptide (384-well low-volume plate)

Read speed

96 wells: 30 s
384 wells: 1 min 50 s
1536 wells: 7 min 20 s

Reagent Dispensers

Supported detection modes

All modes

Number

2 syringe pumps

Supported labware

6- to 384-well plates, Petri dishes

Dead volume

1.1 mL, with backflush

Dispense volume

5-1,000 µL in 1 µL increments

Dispense accuracy

±1 µL or 2%

Dispense precision

≤2% at 50-200 µL

Physical characteristics

Power rating

24VDC

Power consumption

250W max.

Dimensions (W x D x H)

Without TRF laser:
15.4 x 20.7 x 16.1 in (39.2 x 52.5 x 40.8 cm)
With TRF laser:
15.4 x 24.2 x 16.1 in (39.2 x 61.4 x 40.8 cm)

Weight

Without TRF laser: 78 lb (35.4 kg)
With TRF laser: 100 lb (45.3 kg)