Manufacturer

Spectramax

Model 

M2e

Serial No. 

DE 05270

Wavelength range

200-1000 nm

Wavelength selection

Monochromator, tunable in 1 nm increments

Wavelength bandwidth

≤ 4.0 nm full width half maximum

Wavelength accuracy

±2.0 nm across wavelength range

Wavelength repeatability

±0.2 nm

Photometric range

0.000 to 4.000 OD

Photometric resolution

0.001 OD

Photometric accuracy linearity (plate), 0-2.0 OD

< ± 1.0% and ± 0.006 OD

Photometric precision (repeatability)

< ± 1.0% and ± 0.003 OD

Stray light

≤ 0.05% at 230 nm

Photometric stabilization

Instantaneous

Photometric drift

None (continuous referencing of monochromatic input)

Calibration

Automatic before every endpoint read and before the first kinetic read

Optical alignment

None required

Lightsource

Xenon flash lamp (50 Watts)

Average lamp lifetime

1 billion flashes

Illumination

Top down (plates); horizontal (cuvettes)

Photodetector

Silicon photodiode

Endpoint baseline noise (cuvette)

± 0.003 OD @190, 405, 850 nm

Endpoint kinetic noise (cuvette)

± 0.003 OD @190, 405, 850 nm

≥ 0.2 mOD/min and ≤ 0.2 mOD/min

Sensitivity

Top Read (M2 and M2e)

3.0 fmol/well in 200 µL FITC (96 well microplate and 15 pM)

3.0 fmol/well in 75 µL FITC (384 well microplate and 40 pM)

Bottom Read (M2e only)

5.0 fmol/well in 200 µL FITC (96 well microplate and 25 pM)

5.0 fmol/well in 75 µL FITC (384 well microplate and 67 pM)

Sensitivity (cuvette)

< 15 pM fluorescein

Excitation wavelength range

250-850 nm

Emission wavelength range

250-850 nm (M2e)

Wavelength selection

Monochromators, tunable in 1 nm increments

Bandwidth (excitation, emission)

9 nm, 9 nm

Number of excitation/emission pairs per plate

4

Dynamic range 106

10^6 in 96-well black plates: auto gain circuitry

System validation

Self-calibrating with built-in fluorescence calibrators

Lightsource

Xenon flash lamp (1 joule/flash)

Average lamp lifetime

1 billion flashes

Detector

Photomultiplier (R3896)

Sensitivity

0.5 fmol/well Eu-Ch

Wavelength range

250-850 nm

Wavelength selection

Monochromators, tunable in 1 nm increments

Bandwidth (excitation, emission)

9 nm, 9 nm

Precision data collection

1–100 flashes; delay of 0–600 µs before read; integration time selectable 50–1500 µs

Sensitivity

10 amol/well alkaline phosphate 200 μL

Wavelength range

250-850 nm

Crosstalk

< 0.5% in 96- and 384-well microplates

Front Panel Operation

Single wavelength Absorbance, %Transmittance, Fluorescence reading of the cuvette (or test tube)

Using SoftMax Pro Software

Express data as Absorbance, %Transmittance, Fluorescence, Luminescence

Single wavelength reading of microplate and/or cuvette

Multiple wavelength (up to four) reading of microplate or cuvette

Kinetic and kinetic graphics of microplate and/or cuvette

Spectral scan (190–1000 nm) of microplate and/or cuvette

Well scan of microplate using absorbance or fluorescence intensity

Microplate read time (endpoint), Standard read

96 wells in 24 seconds (single wavelength, absorbance)

96 wells in 15 seconds (single wavelength, fluorescence intensity)

384 wells in 1:57 minutes (single wavelength, absorbance)

384 wells in 45 seconds (single wavelength, fluorescence intensity)

Microplate read time (endpoint), Standard read with PathCheck Pathlength Measurement Technology

96 wells in 2:07 minutes (single wavelength, absorbance)

384 wells in 7:19 minutes (single wavelength, absorbance)

Microplate read time (endpoint), Speed read

96 wells in 18 seconds (single wavelength, absorbance)

384 wells in 49 seconds (single wavelength, absorbance)

Cuvette (Normal scan)

45*K nm/min, where K = wavelength interval

Cuvette (Speed scan)

130*K/min, where K = wavelength interval

Wavelength range

250-850 nm

Crosstalk

< 0.5% in 96- and 384-well microplates

Reading chamber

Isothermal when temperature regulation is not enabled

Range

4°C above ambient to 45°C when temperature regulation enabled.

Note: To achieve temperature regulation at 45°C, the ambient temperature must be > 20°C.

Resolution

Resolution ± 0.1°C

Accuracy

± 1.0°C for microplate and cuvette chamber

Temperature uniformity at equilibrium

± 0.5°C at 37°C

Chamber warmup time

15–30 minutes (measured on air) after initiation of temperature regulation

Temperature regulation

4 sensors

Drift

± 0.2°C (regulated)

Temperature regulation diagnostics

Temperature regulation system is continuously monitored and updated

Evaporation

Plate lid required to minimize evaporative cooling