- Applied Biosystems 7900HT Fast Real-Time PCR qPCR Thermal Cycler
- 384 Well Block installed
Dyes for calibration, which the customer will need a regular source for on a calibration cycle every 6 months.
After its initial disinfection and inspection, our technicians tested the 7900HT with the following results:
- Powered on; no beeps or excessive noise: PASSED
- Connected with the software: PASSED
- Able to open and close the door: PASSED
The customer needs the dye and needs to calibrate the machine before first use occurs. Laser Counter reads error; therefore, we cannot say what the hours are on the laser.
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Manufacturer
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Applied Biosystems
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Model
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7900HT
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Serial No.
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200736
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Mfg. Date
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September 2002
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Calibrated Dye
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SYBR, Green I, ROX, NED, JOE, TAMRA, VIC, FAM, TET
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Capacity
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Up to 84 x 384-well plates with optional automation accessory
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Detection Method
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SYBR, Primer-Probe Detection
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Format
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96-well plate
(optional 384-well plate with block installed)
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High-throughput Compatibility
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High-throughput Compatible, Multiplexing
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Optics
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Extended-life 488 nm argonian laser excitation source, No filters (CCD acts as spectrograph with continuous detection 500-650 nm)
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Passive Reference Dye
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ROX (Separate Tube), No ROX, Other Passive Reference Dye, ROX (Pre-mixed)
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Reaction Speed
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Fast
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Reaction Volume Range
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5 - 20 ul
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Run Time
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52 minutes (384-well in fast mode), <2 hrs (384-well in standard mode)
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Sample Ramp Rate
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Fast mode: +/- 3°C/sec
Standard mode: +/- 1.6°C/sec
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Temperature Accuracy
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+/- 0.25°C of display temperature
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Temperature Range
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4-100°C
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Temperature Uniformity
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+/- 0.5°C
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Thermal Cycling System
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Peltier-Based System
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Throughput
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>5,000 real-time PCR rxns per day (384-well block and automation accessory), >10,000 endpoint PCR rxns per hour (384-well block and automation accessory)
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Sensitivity
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Up to 10 copies
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Dynamic Range
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Up to 9 logs of linear dynamic range
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Power Requirements
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200-240 VAC, 16A, 50/60Hz
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Dimensions (W x D x H)
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21 x 30 x 25 in.
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