- Applied Biosystems 7900HT Fast Real-Time PCR qPCR Thermal Cycler
- 384 well block installed
- Dyes for calibration which the customer will need a regular source for on a calibration cycle every 6 months
After its initial disinfection and inspection, our technicians tested the 7900HT with the following results:
- Powered on, no beeps or excessive noise: PASSED
- Connected with the software, able to open and close the door: PASSED
- The customer needs the dye and needs to calibrate the machine before first use occurs. Laser Counter reads error and therefore we cannot say what the hours are on the laser
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Manufacturer
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Applied Biosystems
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Model
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7900HT
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Serial No.
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200736
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Mfg. Date
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September 2002
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Calibrated Dye
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SYBR, Green I, ROX, NED, JOE, TAMRA, VIC, FAM, TET
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Capacity
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Up to 84 x 384-well plates with optional automation accessory
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Detection Method
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SYBR, Primer-Probe Detection
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Format
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96-well plate
(optional 384-well plate with block installed)
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High-throughput Compatibility
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High-throughput Compatible, Multiplexing
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Optics
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Extended-life 488 nm argonian laser excitation source, No filters (CCD acts as spectrograph with continuous detection 500-650 nm)
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Passive Reference Dye
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ROX (Separate Tube), No ROX, Other Passive Reference Dye, ROX (Pre-mixed)
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Reaction Speed
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Fast
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Reaction Volume Range
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5 - 20 ul
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Run Time
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52 minutes (384-well in fast mode), <2 hrs (384-well in standard mode)
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Sample Ramp Rate
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Fast mode: +/- 3°C/sec
Standard mode: +/- 1.6°C/sec
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Temperature Accuracy
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+/- 0.25°C of display temperature
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Temperature Range
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4-100°C
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Temperature Uniformity
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+/- 0.5°C
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Thermal Cycling System
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Peltier-Based System
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Throughput
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>5,000 real-time PCR rxns per day (384-well block and automation accessory), >10,000 endpoint PCR rxns per hour (384-well block and automation accessory)
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Sensitivity
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Up to 10 copies
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Dynamic Range
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Up to 9 logs of linear dynamic range
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Power Requirements
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200-240 VAC, 16A, 50/60Hz
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Dimensions (W x D x H)
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21 x 30 x 25 in.
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