You Will Receive
- Becton Dickinson FACSCalibur Flow Cytometer
- Cytek DxP Multi-Color
- PC keyboard
- Mouse
- Power cable
Note: This does not include a monitor but it can be used with any Standard VGA monitor.
Why is this being sold “For-Parts or Repair”?
We were not able to get the PC to acknowledge it when it was connected. We used a USB to serial adapter with only one serial port but lack knowledge of how the connection is to be configured. There is a good chance that this can be brought back into working condition by someone with a little experience with the FACSCaliber/Cytek system and software.
Specifications
Cytometer Specifications
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Manufacturer
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Becton Dickinson
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Model
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FACSCalibur
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Serial No.
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1272
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Mfg. Date
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2003
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Dimensions (W x D x H)
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Sensor Module
36 in x 24.2 in x 26.5 in / 49 in with cover open
Computer: 48 x 41 x 54 cm (19”L x 16”D x 23”H)
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Weight
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Sensor Module: 109.1 kg (240 lbs) Computer: 50 kg (110 lbs)
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Fluidics
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General Operation
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Front key panel control provides three modes: RUN, STNDBY, and PRIME; automatic standby mode conserves sheath fluid by stopping sheath flow when no sample tube is installed.
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Fluid Reservoirs
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Easily accessible 4-L capacity sheath and waste containers are housed in a convenient pull-out drawer; level detectors automatically indicate low levels of sheath or high levels of waste.
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Sample Flow Rates
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Three selectable flow rates of 60 µL/min, 35 µL/min, and 12 µL/min. Pressure difference between sheath and sample is regulated and monitored; particle velocity in flow cell is approximately 6 meters/second.
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Quartz Cuvette
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Internal cross-section is rectangular 430 µm x 180 µm; external surfaces are anti-reflection coated for maximum transmission of laser light
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Sample Concentration
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Single-cell suspension of 105 to 2 x 107 particles/mL recommended range
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Signal Processing
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Workstation Resolution
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1024 channels on all parameters
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Dynamic range
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Logarithmic amplifiers for SSC, FL1, FL2, FL3, and FL4 (with FL4 option) provide four log decade range
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Fluorescence Compensation Networks
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Fluorescence spectral overlap can be compensated between FL1 and FL2, between FL2 and FL3 channels, and between FL3 and FL4 (with FL4 option)
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Pulse Processing
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Width and Area measurements for discriminating doublets; available for all fluorescence parameters
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Time
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Time available correlated to any parameter for kinetic experiments or other applications.
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Sample Loading Specifications
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Sample delivery
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Tube-lifter design with multiple sensors which verify rack identification and tube position
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Rack Capacity
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40 (12 x 75-mm) tubes per rack
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Rack Support
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Up to 16 racks per FACS Loader
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Data Entry
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Sample information, reagent panels, and rack information can be defined for up to 640 tubes (40 tubes x 16 racks) at a time
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Loader Control
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Automated control through WorklistManager software and manual control with stat interrupt capability through FACS Loader electronic keypad
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Mixing Mode
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Adjustable High Energy and Low Energy mix
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Sorting Specifications
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Sorting Purity
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>95%
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Capture Rate
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300 cells/second
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Sort Modes
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Three modes (all aerosol-free): single cell, exclusion, and recovery
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Recovery
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Depends upon sample and sorting conditions, >50%
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Sterile Sorting
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System design allows for aerosol-free sterile sorting
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