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Manufacturer
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BioTek Instruments, Inc
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Model
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EL406
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Ref. No.
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406PUB1
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Serial No.
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19010415
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General
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Microplate types
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96- and 384--well Low profile and standard height Solid and filter bottom (option)
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Onboard software
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Create, edit, or run multiple protocols
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Software (computer control)
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Agilent BioTek Liquid Handling Control (LHC2) software Agilent BioTek LHC2 Secure software enables 21 CFR Part 11 compliance (option) Agilent BioTek LHC SiLA compliant driver (option)
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Separation
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Biomagnetic separation, vacuum filtration (optional)
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Shaking
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Programmable up to 60 minutes Slow, medium, fast, or variable
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Soaking
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Programmable up to 60 minutes
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Ultrasonic advantage
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Standard on most configuration
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Automation
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Agilent BioTek BioStack microplate stacker and third-party automation compatible Agilent BioTek BioSpa 8 automated incubator compatible Agilent BenchCel microplate handler compatible
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Washing
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Manifold types
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96-well washing: 96-tube manifold−straight dispense tubes 96- and 384-well washing: 96-tube Dual-Action manifold−20° angled dispense tubes 384-well washing (fast): 192-tube Dual-Action manifold−7° angled dispense tubes
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Volume range
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3-3,000 µL/well, in 1 µL increments
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Wash cycles
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1-250
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Buffer/reagent selection
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Auto switching module for up to four buffers (option)
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Supply bottle
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4 L or 10 L (optional)
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Dispense accuracy
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±3%
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Dispense precision
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≤3% CV (model dependent)
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Residual volume
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≤2 µL/well
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Wash speed
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96 wells, 300 µL/well, 96-tube manifold: 13 s 384 wells, 100 µL/well, 192-tube manifold: 17 s
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Flow rates
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High flow to low flow Optimized rates for cell assays
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Sterilization
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Chemical
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Vacuum range for filtration
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Selectable: 0 to -380 mmHg (final at 30 s) Filtration time: 5−999 s
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Dispensing - Peristaltic Pump (Multichannel)
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Manifold types
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8-tip (1 x 8) cassette with plastic, 316 SS tubes or sapphire-jeweled 316 SS tubes
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Fluid delivery
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1 peristaltic pump
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Dispense speed
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96 wells, 10 µL/well: 8 s 384 wells, 5 µL/well: 12 s
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Volume range
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500 nL to 3,000 µL/well, selectable in 1 µL increments
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Flow rates
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User programmable rates from high to low Optimized rates for cell assays
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Dispense Performance
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Recommended volume range
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1 µL cassette: 1−50 µL 5 µL cassette: 5−2,500 µL 10 µL cassette: 10−3,000 µL
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Dispense accuracy
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1 µL cassette: ± 5% at 1 µL 5 µL cassette: ± 2.0% at 5 µL 10 µL cassette: ± 2.0% at 10 µL
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Dispense precision
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1 µL cassette: ≤ 5% CV at 1 µL; ≤ 10% CV at 500 nL 5 µL cassette: ≤ 2.5% CV at 5 µL 10 µL cassette: ≤ 2.0% CV at 10 µL
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Minimum prime volume
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1 µL cassette: 1.20 mL 5 µL cassette: 4.23 mL 10 µL cassette: 7.36 mL
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Recommended cassette replacement interval
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1 µL cassette: 1,000 384-well microplates at 5 µL/well 5 µL cassette: 1,000 96-well microplates at 50 µL/well 10 µL cassette: 1,000 96-well microplates at 100 µL/well
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Sterilization
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Autoclave, chemical
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Dispensing - Syringe Pump
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Manifold types
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96-well dispensing: One 16-tube (2 x 8) manifold−316 SS tubes
96-/384-well dispensing: Two 16-tube (1 x 16) manifolds−316 SS tubes
1536-well dispensing: Two 32-tube (1 x 32) manifolds−sapphire-jeweled 316 SS or 316 stainless steel tubes
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Fluid delivery
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Two positive displacement syringe drives
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Dispense speed
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20 µL/well, 96 wells, 1 x 16 tubes: 5.25 s 20 µL/well, 384 wells, 1 x 16 tubes: 14 s 3 µL/well 1536 wells, 2 x 32 tubes: 7 s
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Volume range
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3−3,000 µL/well, selectable in 1 µL increments Minimum prime volume: 12 mL
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Flow rates
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User-programmable rates from high to low
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Dispense accuracy
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± 1 µL at 5 µL ± 1 µL at 20 µL ± 1% at 100 µL
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Dispense precision
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≤ 5% CV at 5 µL ≤ 2.5% CV at 20 µL ≤ 1% CV at 100 µL
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Supply bottle
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1 L or 2 L
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Sterilization
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Chemical, autoclavable option
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Physical Characteristics
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Power rating
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100 to 240V~, 50/60Hz, 8.0A
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Accessory outlet
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≤5A, used for vacuum pump
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Dimensions (W x D x H)
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16 x 18 x 12.5 in (42 x 46 x 32 cm)
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Weight
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32 lb (14.5 kg)
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