- Molecular Devices SpectraMax iD5 Multi-Mode Microplate Reader
- Computer with SoftMax Pro 7 Software Installed and Licensed
- Monitor, Keyboard, and Mouse
- Tools and Transport Lock
- VGA Cable
- Ethernet Cable
- x3 Power Cables
- 90-Day Warranty
- 350 nm / 60 nm bandwidth
- 490 nm / 10 nm bandwidth
- 616 nm / 10 nm bandwidth
- FP H polarizer
- FP V polarizer
After its initial disinfection and inspection, our technicians tested the Molecular Devices SpectraMax iD5 multi-mode microplate reader with the following results:
- Reader power and initialization: PASSED
- Reader and computer connection: PASSED
- Touchscreen user interface: PASSED
- Plate loading and unloading: PASSED
Notice: Transport locks will be installed when this instrument arrives at your location.
|
Manufacturer
|
Molecular Devices
|
|
Model
|
SpectraMax iD5
|
|
Serial No.
|
37 570-3022
|
|
Mfg. date
|
2017-11-15
|
|
Plate formats
|
6 to 384-well plates
|
|
Light source
|
Xenon flash lamp
|
|
Reading capability
|
Microplates, cuvettes (via adapter) SpectraCuvette Adapters without a sticker have a plate height of 24 mm and cannot be used in the SpectraMax iD3 or SpectraMax iD5.
|
|
Detectors
|
Photomultiplier tube and photodiode
|
|
Shaking
|
Linear, orbital and double orbital
|
|
Temperature control
|
5°C (7.2°F) above ambient up to 66°C (150.8°F)*
|
|
Temperature uniformity
|
±0.75°C (1.35°F)
|
|
Temperature accuracy
|
±1°C (1.8°F) at 37°C (98.6°F) set point
|
|
Spectral scanning
|
Abs, Fl, Lum, TRF
|
|
Endpoint reading
|
Abs, Fl, Lum, TRF, FP, FRET, TR-FRET
|
|
Kinetic reading
|
Abs, Fl, Lum, TRF, FP, FRET, TR-FRET
|
|
Well scanning
|
Over 20 by 20
|
|
Wavelength selection
|
1.0 nm increments
|
|
Robotic compatible
|
Yes
|
|
Power rating
|
100-240VAC, 50/60Hz, 2A
|
|
Dimensions (W x D x H)
|
20.94 x 23.54 x 15.79 in (53.2 x 59.8 x 40.1 cm)
|
|
Weight
|
88.1 lb (40 kg)
|
*For >66°C, minimum 25°C ambient temperature is required.
Read times (minutes:seconds)
|
Read Mode
|
96 wells
|
388 wells
|
|
Absorbance
|
0:28
|
1:28
|
|
Fluorescence intensity*
|
0:21
|
1:08
|
|
Luminescence*
|
0:21
|
1:03
|
|
Time-resolved fluorescence
|
0:25
|
1:12
|
|
Fluorescence polarization
|
0:35
|
1:48
|
*Using the lowest setting and speed read when available.
Absorbance photometric performance:
|
Wavelength range
|
230 - 1000 nm
|
|
Wavelength bandwidth
|
4.0 nm full width half maximum
|
|
Wavelength accuracy
|
±2.0 nm across wavelength range
|
|
Wavelength repeatability
|
±1.0 nm
|
|
Photometric range
|
0 - 4.0 OD
|
|
Photometric resolution
|
0.001 OD
|
|
Photometric accuracy
|
< ±0.010 OD ±1.0%
0 - 3 OD VIS, 0 - 3 OD UV
|
|
Photometric precision (repeatability)
|
< ±0.003 OD ±1.0%
0 - 3 OD VIS, 0 - 3 OD UV
|
|
Stray light
|
≤ 0.05% @ 260 nm, 280 nm
|
Fluorescence intensity performance:
|
Wavelength range (EX mono)
|
250-830 nm
|
|
Wavelength range (EM mono)
|
270-850 nm
|
|
Wavelength range (filters)
|
See accessory list
|
|
Wavelength selection (mono)
|
1.0 nm increments
|
|
Dynamic range
|
>6 logs
|
|
Optimized top sensitivity (fluorescein)
|
|
|
96 wells
|
1 pM - Monochromator
0.3 pM - Filters
|
|
384 wells
|
1 pM - Monochromator
0.5 pM - Filters
|
|
Optimized bottom sensitivity (fluorescein)
|
|
|
96 wells
|
2 pM - Monochromator
2 pM - Filters
|
|
384 wells
|
2.5 pM - Monochromator
2.5 pM - Filters
|
Fluorescence polarization performance:
|
Wavelength range (EX mono)
|
(300-)* 400-750 nm, 1.0 nm increments
|
|
Wavelength range (EM mono)
|
(300-)* 400-750 nm, 1.0 nm increments
|
|
Wavelength range (filters)
|
See accessory list
|
|
Detection limit**
|
|
|
96 wells black
|
2 mP @ 10 nM - Monochromator
1 mP @ 1 nM - Filters
|
|
384 wells black
|
2 mP @ 10 nM - Monochromator
2 mP @ 1 nM - Filters
|
|
Measurement range***
|
Delta 200 mP - Monochromator
Delta >320 mP - Filters
|
*Requires optional UVIS polarizer
**1x Stdev Fluorescein replicates [mP]
***ThermoFisher P3088, FP One-Step reference kit
Luminescence performance:
|
Wavelength range
|
300 - 850 nm
300 - 650 nm for “All Wavelengths” setting
|
|
Wavelength selection
|
Choice of simultaneous detection of All Wavelengths or selection in 1.0 nm increments
|
|
Dynamic range
|
>7 decades
|
|
Crosstalk
|
<0.1% in white 96- and <0.2% in white 384-well microplates
|
|
Detection limit
|
20 amol ATP (“Flash” luminescence using Promega ENLITEN ATP Assay System)
|
Time-resolved fluorescence performance:
|
Wavelength range (EM mono)
|
450-750 nm
|
|
Wavelength range (EX filter)
|
350 nm
|
|
Wavelength range (EM filter)
|
490 nm (Terbium), 616 (Europium)
For other filters, please see accessory list
|
|
Linear dynamic range
|
Up to 5 logs
|
|
Detection limit
|
|
|
Standard TRF
|
30 fM Europium (6 amol/well) - 96 wells (white)
30 fM Europium (3 amol/well) - 384 wells (white)
|
|
Enhanced TRF Module (optional)
|
10 fM Europium (2 amol/well) - 96 wells (white)
10 fM Europium (1 amol/well) - 384 wells (white)
|
