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Manufacturer
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BioTek Instruments, Inc
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Model
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Synergy Neo2
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Ref. No.
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NEO2S
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Serial No.
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1908073
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Mfg. date
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2019-08-07
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Detection mode
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Monochromators: FL, Lum, UV-Vis Abs, TRF (secondary)
Filters: FL, TRF, FP, Lum, Alpha
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Read mode
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End point, kinetic, spectral scanning, well area scanning
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Microplate types
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1- to 1536-well plates
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Other labware
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Compatible with Take3 Micro-Volume Plates with 2 µL microspots
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Temperature control
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3°C above ambient to 65°C with Condensation Control
Variation ±0.2°C at 37°C
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Shaking
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Linear, orbital, double orbital
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Software
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Gen5 Data Analysis Software included
Gen5 Secure Software option for 21 CFR Part 11 compliance features
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Automation
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BioStack and 3rd party compatible
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CO2 and O2 control
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0-20% CO2 control and 1-19% O2 control, with optional Gas Controller
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Barcode reader
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Multi-directional, 1D and 2D camera-based scanner
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Read height
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Auto Z, 0.1 mm steps, top/bottom (Filters), top (Mono)
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Kinetic speed
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96-well: 6 seconds
384-well: 11 seconds
1536-well: 25 seconds
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With stacker, minimum processing time per plate
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96-well: 20 seconds
384-well: 25 seconds
1536-well: 39 seconds
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Absorbance
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Light source
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Xenon flash lamp
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Wavelength selection
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Monochromator
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Wavelength range
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230-999 nm, in 1 nm increments
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Bandwidth
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2 nm (230-285 nm), 4 nm (>285 nm)
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Dynamic range
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0-4.0 OD
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Resolution
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0.0001 OD
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Fluorescence Intensity
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Sensitivity
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Filter cubes:
Fluorescein 0.2 pM (384-well low-volume plate) - top
Fluorescein 1 pM (1536-well plate) - top
Fluorescein 1 pM (384-well plate) - bottom
Quad Monochromator:
Fluorescein 2 pM (384-well low-volume plate) - top
Fluorescein 2.5 pM (384-well plate) - bottom
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Light source
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High energy xenon flash lamp
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Read height
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Auto Z, 0.1 mm steps, top/bottom (filters), top (mono)
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Wavelength selection
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Double grating monochromators (top/bottom)
Filter cubes (top/bottom)
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Wavelength range
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Monochromators: 250-850
Filter cubes - dual PMT: 200-850
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Monochromator bandwidth
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Variable, from 3 nm to 50 nm, in 1 nm increment excitation/emission
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Detection system
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Single PMT or dual PMTs (top filter system)
Low Noise PMT bottom filter system
Red shifted PMT top/bottom monochromator system
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Luminescence
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Sensitivity
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5 amol ATP (flash) (384-well low-volume plate)
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Wavelength range
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300-700 nm
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Dynamic range
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>6 decades
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Fluorescence Polarization
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Sensitivity
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1 mP standard deviation at 1 nM fluorescein (384-well low volume plate)
1.5 mP standard deviation at 1 nM fluorescein (1536-well plate)
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Light source
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Xenon flash lamp
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Wavelength selection
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Filter cubes (top/bottom)
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Wavelength range
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280-850 nm
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Detection system
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Single PMT or dual PMTs
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Time-Resolve Fluorescence
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Light source
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Xenon flash lamp
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Sensitivity
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Europium 40 fM (384-well low volume plate)
Europium 70 fM (1536-well plate)
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Wavelength selection
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Filter Cubes (top/bottom)
Double grating monochromator (top/bottom)
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Wavelength range
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Monochromators: 250-850 nm
Filters: 200-850 nm
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Detection system
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Single PMT or dual PMTs
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Alpha Detection
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Sensitivity
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100 amol LCK peptide (384-well low-volume plate)
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Light source
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100 mW 680 nm laser
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Detector
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PMT
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Wavelength selection
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Filter cubes
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Read speed
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96-well: 30 seconds
384-well: 1 minute 50 seconds
1536-well: 7 minutes 20 seconds
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Reagent Dispensers
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Number
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2 syringe pumps
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Dispense volume
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5-1000 µL, in 1 µL increments
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Dead volume
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1.1 ml, 100 µL with back flush
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Plate geometry
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6- to 384-well microplates, Petri dishes
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Dispense precision
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≤2% at 50-200 µL
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Dispense accuracy
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±1 µL or 2%
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Physical characteristics
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Power consumption
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250 Watts max.
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Dimensions (W x D x H)
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15.4 x 20.7 x 16.1 in (39 x 52.5 x 41 cm)
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Weight
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78 lb (35 kg)
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