Thermo Scientific EASY-nLC 1200 System LC140 Nanoflow Liquid Chromatography

Manufacturer

Thermo Scientific

Model

EASY-nLC 1200

Part No.

LC140

Serial No.

LC-031124

Pressure range

0-1200 bar

Flow range (gradient)

20-2000 nL/min
Recommended: 100-1000 nL/min

Flow range (loading & equilibration)

≤25 µL/min

Retention time reproducibility

0.1-0.4% RSD within recommended flow range

Pick-up volume range

0.10-18.00 µL (20 µL loop)

Injection reproducibility

≤0.2% RSD at 5 µL pick-up
≤3.0% RSD at 0.1 µL pick-up

Injection linearity

R ≥ 0.9985 for 0.5–10 µL injection volume
R ≥ 0.9995 for 0.3–1.6 µL injection volume

Carry-over

<0.05% (caffeine)
<0.1% for m/z 722.3 and <1.00% in general (100 fmol BSA digest, YIC*DNQDTISSK)

Gradient delay volume (mixing tee to venting tee)

<1 µL

Injection loops

20 µL default (5 µL and 50 µL optional)

Gradient and injection pumps

Single action syringe pumps (sapphire piston), 140 µL volume
Gradient pumps: 100 µL/min refill speed
Injection pump: 40 µL/min refill speed

Valves

4 identical 6-port, 3-position proprietary valves from Thermo Fisher Scientific

Solvent compatibility

≤95% acetonitrile in water. Wetted parts: Fused silica, PEEK™, 316 stainless steel, zirconium dioxide

Sample tray formats

6 × 8 HPLC vials
1 × 96 or 1 × 384 MTP and 6 vials
2 × 48 or 4 × 96 PCR strips and 6 vials

Autosampler cooling range

Minimum of 5°C
Maximum of 20°C below ambient

MS drivers

LC Devices 3.0 for XCalibur 3.0 and later

Remote support

Direct internet access for online diagnosis by EASY-nLC technical experts

Operating conditions

5–30°C ambient temperature
20–80% relative humidity

Power rating

100-240 V, 50-60 Hz, 250 W

Dimensions (W x D x H)

14 x 15 x 18 in (36 x 38 x 45 cm)

Weight

82 lb (37 kg)